Research Assistant (Metabolic Liver Disease Lab)
4000 - 6750 SGDFull-time
National University of Singapore
Interested applicants are invited to apply directly at the NUS Career Portal. Please note your application will only be processed if you apply via NUS Career Portal.
NUS Career Portal link:
We regret that only shortlisted candidates will be notified.
Job Description
Purpose of the post
The National University of Singapore invites applications for Research Assistant for the Metabolic Liver Disease Lab in the Department of Medicine, Yong Loo Lin School of Medicine. This role explores functional genomic methods to better understand the molecular mechanisms of metabolic dysfunction-associated fatty liver disease (MAFLD). The RA will get opportunity to collaborate with various teams across the School of Medicine, including pathologists, hepatologists, and data scientists. The ideal candidate will have demonstrated expertise in in vitro mammalian cell-based assays and a keen interest in uncovering the underpinning biological mechanisms of disease.
Job description:
- Assist PI/PF to develop, optimize, and validate in vitro mammalian cell-based assays that are related to metabolic diseases (such us cell culture, RNA/protein extraction qPCR, western blotting and other metabolic-related assays)
- To perform other related duties, incidental to the work described herein (such as conduct data analysis, ensure the validity and reliability of data, maintain accurate and complete records of all findings, present findings to colleagues)
- Assist in Daily lab management tasks such as inventory of supplies and samples, preparing sample processing media and solutions, autoclaving materials, ordering supplies and stocks
- Assist in Laboratory equipment management.
- Assist PI/RF with ethics/grant application
Qualifications
- Minimal education with B.Sc. and/or M.Sc. in Cell Biology, Biochemistry, Molecular Biology, Biomedical or related disciplines with research experience.
- Understanding of the molecular/cellular basis of MAFLD, obesity and T2D, e.g., lipid metabolism dysregulation, insulin resistance, inflammatory pathways) are desired.
- Experience in handling 2D and 3D cell cultures such as hepatocytes, adipocytes or skeletal muscle cells is essential.
- Basic Molecular Biology skills (RNA/protein extraction, qPCR, western blotting) is essential.
- Experience with gene perturbation tools, such as siRNA, shRNA and CRISPR/Cas9 is a strong plus.
- Excellent writing and communication skills in English.
- Highly organized and ability to independent judgement and produce a quality work product within tight time constraint.
- Ability to coordinate and work in a multidisciplinary team.
Applicants should submit the following documents via the NUS Career Portal.
- Cover letter highlighting relevant experience and career goals
- Curriculum Vitae, containing details of three named referees
- A complete NUS Personal Data Consent for Job Applicants (the form is available at NUS Personal Data Consent for Job Applicants)
Vacancy posted 7 days ago
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